irf4-fitc 3e4 antibody (Thermo Fisher)
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Thermo Fisher
irf4-fitc 3e4 antibody
Irf4 Fitc 3e4 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf4+(3e4)+fitc+antibody/annexin+v+fitc+apoptosis+detection+kit/pmc08355159-335-11-30
Average 90 stars, based on 1 article reviews
Irf4 Fitc 3e4 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf4+(3e4)+fitc+antibody/annexin+v+fitc+apoptosis+detection+kit/pmc08355159-335-11-30
Average 90 stars, based on 1 article reviews
irf4-fitc 3e4 antibody - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Altered function and differentiation of age-associated B cells contribute to the female bias in lupus mice"
Article Title: Altered function and differentiation of age-associated B cells contribute to the female bias in lupus mice
Journal: Nature Communications
doi: 10.1038/s41467-021-25102-8
Figure Legend Snippet: RNA-seq was performed on sorted CD11c + and CD11c − GL7 + CD38 lo B cells (gated on B220 + splenocytes) from aged (24+wk) female DKO (F) mice. a Volcano plot showing genes differentially expressed ( p < 0.01 after Benhamini–Hochberg false discovery rate (FDR) was used to correct for multiple comparisons) between CD11c + B220 + GL7 + CD38 lo (CD11c + ) and CD11c − B220 + GL7 + CD38 lo (CD11c − ) cells. b Plot showing the enrichment of the ABC geneset from DKO mice in CD11c + B220 + GL7 + CD38 lo cells. c Heatmap showing the expression of GC B cell target genes in CD11c + and CD11c − B220 + GL7 + CD38 lo cells. d Representative plots and quantifications of pre-GC B cells (BCL6 mid IRF4 + ) and GC B cells (BCL6 hi IRF4 − ) among CD11c + CD19 + GL7 + Fas + (CD11c + ; red) and CD11c − CD19 + GL7 + Fas + (CD11c − ; black) cells from aged (24+wk) DKO(F) mice. Data show mean ± SEM; n = 6 from 2 independent experiments; p -value by paired two-tailed t -tests. e Plot showing the enrichment of a pre-GC geneset (GSE12845) in CD11c + B220 + GL7 + CD38 lo cells. f Representative histograms and quantifications of the phosphorylation of SYK(Y352) and LYN(Y416) in CD11c + CD19 + GL7 + Fas + cells (CD11c + ; red), CD11c − CD19 + GL7 + Fas + (CD11c − ; black) cells from aged (24+wk) DKO(F) mice. CD19 + CD11c + CD11b + (ABCs; green) CD19 + GL7 − Fas − cells (gray) are shown as control. Data show mean ± SEM; n = 4 from 3 independent experiments; p -value by paired two-tailed t -test. g Plot showing the top pathways upregulated in CD11c − GL7 + CD38 lo cells (black) and CD11c + GL7 + CD38 lo cells (red) by GSEA. Dotted line indicates significance threshold at FDR q < 0.25. h Representative histogram and quantification of Ki67 expression in the indicated populations from DKO(F) mice. Data show mean ± SEM; n = 5 from 3 independent experiments; p -value by paired two-tailed t -tests. i Heatmap showing the expression of genes related to apoptotic cell clearance in CD11c + and CD11c − GL7 + CD38 lo cells from DKO ( F) mice. j Representative histogram and quantification of MerTK expression on the indicated populations from aged (24+wk) DKO(F) mice. Data show mean ± SEM; n = 8 from 4 independent experiments; p -value by paired two-tailed t -tests.
Techniques Used: RNA Sequencing, Expressing, Two Tailed Test, Phospho-proteomics, Control
Figure Legend Snippet: a RNA-seq was performed on sorted CD11c + and CD11c − PB/PCs (CD138 + TACI + ) from aged (24+wk) female DKO (F) mice. Volcano plot showing differentially expressed genes ( p < 0.01 after Benhamini-Hochberg false discovery rate (FDR) was used to correct for multiple comparisons) between CD11c + and CD11c − PB/PCs. Blue dots indicate ABC target genes upregulated in CD11c + PB/PCs. b Plot showing the enrichment of the ABC geneset from DKO mice in CD11c + PB/PCs. c Representative histograms and quantifications of Fcrl5, Cxcr3, and Tbet in CD11c + PB/PCs (CD19 mid/lo CD138 + ; red ) and CD11c − PB/PCs (black) from aged (24+wk) DKO(F) mice. CD19 + CD11c + CD11b + (ABCs) (green) and CD19 + GL7 − Fas − cells (gray) are shown as controls. Data show mean ± SEM; n = 6 for Tbet, n = 8 for CXCR3, n = 9 for FcRL5 over 3–5 independent experiments; p -value by paired two-tailed t -tests. d Plots showing the enrichment of Blimp1-induced and IRF4-induced target genes in CD11c − PB/PCs by GSEA. e Representative histograms and quantifications of B220, MHC-II, and Ki67 expression in the indicated populations from aged (24+wk) DKO(F) mice. Data show mean ± SEM; n = 4 for Ki67, n = 5 for B220, n = 7 for MHC-II over 2–3 independent experiments; p -value by paired two-tailed t -tests. f Representative RT-qPCR showing Ciita expression in CD11c + or CD11c − PB/PCs sorted from aged (24+wk) DKO(F) mice as in Fig. 6a. Data representative of 3 independent experiments and show mean ± SD; n = 3; p -value by unpaired two-tailed t -test. g Plot showing the top pathways enriched in CD11c + PB/PCs as compared to CD11c − PB/PCs by GSEA. Dotted line indicates significance threshold at FDR q < 0.25. h , i Heatmap showing the expression of migratory ( h ) and inflammatory target genes ( i ) that are differentially expressed ( p < 0.01) between CD11c + and CD11c − PB/PCs in DKO(F) mice from Fig. 6a. j Representative RT-qPCR showing Il1b expression in CD11c + or CD11c − PB/PCs sorted from aged (24+wk) DKO(F) mice as in Fig. 6a. Data representative of 3 independent experiments and show mean ± SD; n = 3; p -value by unpaired two-tailed t -test.
Techniques Used: RNA Sequencing, Two Tailed Test, Expressing, Quantitative RT-PCR
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Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Phospho-proteomics:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Recombinant:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Staining:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Cell Isolation:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: RNA Sequencing:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Software:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Purification:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Binding Assay:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Blocking Assay:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Virus:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Derivative Assay:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Adjuvant:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Protein Extraction:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Electron Microscopy:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Sterility:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Enzyme-linked Immunosorbent Assay:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: Fluorescence:Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was carried out at room temperature for 25 min with the Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants Article Snippet: Article Title: Organoid Polymer Functionality and Mode of Klebsiella Pneumoniae Membrane Antigen Presentation Regulates Ex Vivo Germinal Center Epigenetics in Young and Aged B Cells Article Snippet: Anti-mouse monoclonal antibodies targeting intracellular antigens included anti-H3k27me3 (AF488, AF647; clone C36B11; Cell Signaling), Article Title: External signals regulate germinal center fate-determining transcription factors in the A20 lymphoma cell line. Article Snippet: Due to the apoptosis-prone nature of primary germinal center B (GCB) cells, it remains a huge challenge to dissect signals that guide their differentiation towards memory B cells and plasma cells in vitro.. Here we show that the murine lymphoma cell line A20 resembles primary GCB cells in expression of GC-specific surface markers and the master transcription factor BCL6 and may serve as a useful system to model certain GCB cell behaviors in vitro.. Using these cells, we found that both CD40 and B cell receptor (BCR) signaling are able to drive BCL6 downregulation, which is a prerequisite of post-GC B-cell differentiation. Article Title: A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques Article Snippet: After permeabilization, intracellular staining was 699 carried out at room temperature for 25 min with the following antibodies: FITC anti-IRF4 (3E4 – 700 Article Title: Autophagy is a critical regulator of memory CD8+ T cell formation Article Snippet: The following antibodies were used for flow cytometry (antibody clone in brackets): CD8 (Ly2) PE/ PE-CY7; CD8 (53-6.7) FITC/eF450/PE-Cy7; CD4 (GK1.5) PE/FITC/APC; TCRβ (H57-597) FITC/PE/ PE-Cy7/APC; CD3 (145-2C11) eF450/APC; CD62L (MEL-14) FITC/PE-Cy7; CD44 (IM7) FITC/PE-Cy7; KLRG1 (2F1) FITC/PE-Cy7; IRF4 (3E4) FITC; EOMES (Dan11mag) PE-Cy7; CD127 (A7R34) FITC/PE/ eF450; Ki-67 (SolA15) eF450; CD24 (M1/69) APC; CD16/32 (93) purified Fc block; Article Title: Tumor cell-derived IL-10 promotes cell-autonomous growth and immune escape in diffuse large B-cell lymphoma Article Snippet: Cells were stained using the following fluorescent-labeled antibodies which were all purchased from BioLegend (unless otherwise specified and clone numbers in brackets) and used at 1:200 dilution (unless otherwise specified): BV605 anti-mouse CD138 (281-2), BV421 antimouse IgD (11-26c.2a), Article Title: Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells Article Snippet: |
